Ukuze kuvavanywe impembelelo yokongeza i-triglyceride ekutyeni kwimveliso yeprotheyini ye-rumen microbial kunye neempawu zokubiliswa kweemazi zeegusha ezindala ezinomsila omncinci, kwenziwe iimvavanyo ezimbini kwi-vitro nakwi-vivo.

Uvavanyo lwe-in vitro: ukutya okusisiseko (okusekelwe kwizinto ezomileyo) kunye noxinzelelo lwe-triglyceride lwe-0, 2, 4, 6 kunye ne-8g / kg kwasetyenziswa njengesiseko, kwaye ijusi ye-rumen yeemazi zeegusha ze-Adult Small Tailed yongezwa, yaza yafakwa kwi-incubator kwi-39 ℃ kangangeeyure ezingama-48 kwi-in vitro.

Uvavanyo lwe-in vivo: Iimazi ezindala ezingama-45 zahlulwe ngokungacwangciswanga zaba ngamaqela ama-5 ngokobunzima bazo bokuqala (55 ± 5 kg).I-glyceryl tributylateye-0, 2, 4, 6 kunye ne-8 g/kg (ngokusekelwe kwizinto ezomileyo) yongezwa kwisidlo esisisiseko, kwaye ulwelo lwesisu kunye nomchamo zaqokelelwa iintsuku ezili-18.

Iziphumo zovavanyo

1). Impembelelo kwixabiso le-pH kunye noxinzelelo lwe-asidi yamafutha aguqukayo

Iziphumo zibonise ukuba ixabiso le-pH le-culture medium lehlile ngokuthe ngqo kwaye ukuxinana kwe-total volatile fatty acids (TVFA), i-acetic acid, i-butyric acid kunye ne-branched chain volatile fatty acids (BCVFA) kuye kwanda ngokuthe ngqo xai-tributyl glycerideyongezwe kwi-substrate. Iziphumo zovavanyo lwe-in vivo zibonise ukuba i-dry matter intake (DMI) kunye nexabiso le-pH yehlile, kwaye ubuninzi be-TVFA, i-acetic acid, i-propionic acid, i-butyric acid kunye ne-BCVFA banda ngokuthe ngqo ngokongezwa kwei-tributyl glycerideIziphumo zovavanyo lwe-in vivo zibonise ukuba i-dry matter intake (DMI) kunye nexabiso le-pH yehlile, kwaye uxinano lwe-TVFA, i-acetic acid, i-propionic acid, i-butyric acid kunye ne-BCVFA lwanda ngokuthe ngqo ngokongezwa kwe-tributyl glyceride.

Iziphumo zovavanyo lwe-in vivo zibonise ukuba i-dry matter intake (DMI) kunye nexabiso le-pH lehlile, kwaye uxinano lwe-TVFA, i-acetic acid, i-propionic acid, i-butyric acid kunye ne-BCVFA lwanda ngokuthe ngqo ngokongezwa kwe-i-tributyl glyceride.

2). Ukuphucula izinga lokuwohloka kwezondlo

Izinga elibonakalayo lokuwohloka kwe-DM, CP, NDF kunye ne-ADF lenyuke ngokuthe ngqo xai-tributyl glycerideyongezwa kwi-substrate kwi-vitro.

3). Ukuphucula umsebenzi we-enzyme eyonakalisa i-cellulose

Ukongezwa kwei-tributyrinI-in vitro yonyusa ngokuthe ngqo imisebenzi ye-xylanase, i-carboxymethyl cellulase kunye ne-microcrystalline cellulase. Uvavanyo lwe-in vivo lubonise ukuba i-triglyceride yonyusa ngokuthe ngqo imisebenzi ye-xylanase kunye ne-carboxymethyl cellulase.

4). Ukuphucula imveliso yeeproteni zeentsholongwane

Uvavanyo lwe-in vivo lubonise ukubai-tributyrinngokomgaqo-nkqubo kwandisa ubungakanani bemihla ngemihla be-allantoin, i-uric acid kunye ne-purine efunxwayo kumchamo, kwaye kwandisa ukwenziwa kwe-rumen microbial nitrogen.

Isiphelo

I-Tributyriniphucule ukwenziwa kweproteni ye-rumen microbial, umxholo we-total volatile fatty acids kunye nomsebenzi we-enzymes ezitshabalalisa i-cellulose, kwaye yakhuthaza ukuwohloka nokusetyenziswa kwezinto ezomileyo, iproteni eluhlaza, i-neutral detergent fiber kunye ne-acid detergent fiber ekutyeni.

Ibonisa ukuba i-triglyceride inefuthe elihle ekuvunweni nasekuvuthweni kweprotheyini ye-rumen microbial, kwaye inokuba nefuthe elihle ekusebenzeni kakuhle kwemveliso yeemazi ezindala.