Ukutshintshwa ngokuyinxenye kwefishmeal ngomgubo weembotyi zesoya (SBM) njengendlela ezinzileyo neyoqoqosho iye yaphononongwa kwiqela leentlobo ezijoliswe ekurhwebeni ngezilwanyana zasemanzini, kuqukwa ne-rainbow trout (i-rainbow trout)Oncorhynchus mykiss). Nangona kunjalo, i-soy kunye nezinye izinto ezisekelwe kwizityalo ziqulethe amanqanaba aphezulu e-saponins kunye nezinye izinto ezichasene nesondlo ezenza i-subacute enteritis ye-distal intestine kwiintlanzi ezininzi zezi ntlanzi. Le meko ibonakala ngokunyuka kwamathumbu emathunjini, ukudumba, kunye nokungahambi kakuhle kwe-morphological ekhokelela ekwehleni kokusebenza kokutya kunye nokukhubazeka kokukhula.

Kwi-rainbow trout, kubandakanywa ne-SBM ngaphezu kwe-20% yokutya kuye kwaboniswa ukuba kubangele i-soy-enteritis, ibeka i-physiological threshold kwinqanaba elinokuthi litshintshwe kwi-standard aquaculture diet. Uphando lwangaphambili luye lwavavanya inani leendlela zokulwa nale enteritis, kubandakanywa ukuguqulwa kwe-gut microbiome, ukulungiswa kwezithako zokususa izinto ezichasene nesondlo, kunye ne-antioxidant kunye ne-probiotic additives. Enye indlela engakhange ihlolwe kukuqukwa kwe-trimethylamine oxide (TMAO) kwisondlo sasemanzini. I-TMAO yi-cytoprotectant yendalo yonke, eqokelelwe kwiintlobo ezininzi njengeprotheni kunye ne-membrane stabilizer. Apha, sivavanya isakhono se-TMAO sokwandisa uzinzo lwe-enterocyte kunye nokucinezela isibonakaliso se-HSP70 esivuthayo ngaloo ndlela silwa ne-enteritis eyenziwe ngesoya kwaye ikhokelele ekonyukeni kokutya okusebenzayo, ukugcinwa kunye nokukhula kwi-trout yamanzi amnandi. Ngapha koko, sivavanya ukuba i-marine fish solubles, umthombo otyebileyo we-TMAO, unokusetyenziswa njengendlela yoqoqosho yokulawula olu songezo, okwenza ukuba isicelo saso sisetyenziswe kwisikali sorhwebo.

I-rainbow trout efamayo (i-Troutlodge Inc.) yayifuyelwe kubunzima bokuqala obuyi-40 g kunye ne-n=15 ngetanki nganye kwiitanki zokunyanga eziphindwe kathathu. Amatanki atyiswa enye yezidlo ezintandathu ezilungiselelwe ngokwesiseko sezakhamzimba ezigaywayo ezibonelela ngama-40% eprotheyini egaywayo, i-15% yamafutha akrwada, kunye nokuhlangabezana nemilinganiselo efanelekileyo ye-amino acid. Ukutya kwakuquka i-fishmeal 40 control (% of dry diet), SBM 40, SBM 40 + TMAO 3 g kg^-1, SBM 40 + TMAO 10 g kg^-1, SBM 40 + TMAO 30 g kg^-1, kunye ne-SBM 40 + 10% ye-solubles yentlanzi. Iitanki zondliwa kabini yonke imihla ukuya kwi-satiation ecacileyo kwiiveki ze-12 kunye ne-fecal, proximate, histological and molecular analysis eyenziwa.

Iziphumo zolu phononongo ziya kuxutyushwa kunye nokusebenziseka kokubandakanya i-TMAO ukwenza ukusetyenziswa okuphezulu kweemveliso zesoya zase-US kwi-salmonid aquafeeds.