IsishwankatheloOlu vavanyo lwenziwe ukuze kufundwe imiphumo ye-diludine ekusebenzeni kokubeka amaqanda kunye nomgangatho wamaqanda kwiinkukhu kunye nendlela yokusebenza kwemiphumo ngokumisela ii-index ze-parameters zamaqanda kunye ne-serum. Iinkukhu ze-ROM ezili-1024 zahlulwe zangamaqela amane ngalinye liquka iikopi ezine zeenkukhu ezingama-64 nganye, Amaqela onyango afumene ukutya okufanayo okusisiseko okongeziweyo nge-0, 100, 150, 200 mg/kg diludine ngokulandelelanayo kangangeentsuku ezingama-80. Iziphumo bezizizo zilandelayo. Ukongezwa kwe-diludine ekutyeni kuphucule ukusebenza kokubeka amaqanda kwiinkukhu, apho unyango lwe-150 mg/kg lwalungcono kakhulu; izinga layo lokubeka amaqanda lenyuswe nge-11.8% (p< 0.01), ukuguqulwa kobunzima beqanda kwehliswe nge-10.36% (p< 0 01). Ubunzima bamaqanda bonyuswe ngokwanda kwe-diludine eyongeziweyo. I-Diludine yehlise kakhulu uxinzelelo lwe-uric acid kwi-serum (p< 0.01); ukongeza i-diludine kwehlise kakhulu i-serum Ca2+kunye nomxholo we-phosphate ongaphiliyo, kunye nomsebenzi owandisiweyo we-alkine phosphatase (ALP) ye-serum (p< 0.05), ngoko ke ibe nemiphumo ebalulekileyo ekunciphiseni ukuqhekeka kweqanda (p<0.05) kunye nokungaqhelekanga (p< 0.05); i-diludine yonyuse kakhulu ubude be-albumen. Ixabiso le-Haugh (p<0.01), ubukhulu begobolondo kunye nobunzima begobolondo (p< 0.05), i-150 kunye ne-200mg/kg i-diludine nayo yehlise i-choleasterol iyonke kwi-yellow yeqanda (p< 0 05), kodwa yonyusa ubunzima be-yellow yeqanda (p< 0.05). Ukongeza, i-diludine inokunyusa umsebenzi we-lipase (p< 0.01), kwaye yehlise umxholo we-triglyceride (TG3) (p<0.01) kunye ne-cholesterol (CHL) (p< 0 01) kwi-serum, yehlisa ipesenti yamafutha esiswini (p< 0.01) kunye nomxholo wamafutha esibindi (p< 0.01), yayinamandla okuthintela iinkukhu kwisibindi esinamafutha. I-Diludine yandise kakhulu umsebenzi we-SOD kwi-serum (p<0 01) xa yongezwa ekudleni kangangeentsuku ezingaphezu kwama-30. Nangona kunjalo, akukho mahluko ubalulekileyo ufunyenweyo kwimisebenzi ye-GPT kunye ne-GOT ye-serum phakathi kweqela lolawulo kunye nelinyangiweyo. Kwagqitywa kwelokuba i-diludine inokuthintela i-membrane yeeseli kwi-oxidation.
Amagama angundoqoiDiludine; inkukhu; iSOD; icholesterol; i-triglyceride, i-lipase
I-diludine sisixhobo esitsha esingesosondlo sokuncedisa i-oxidation vitamin kwaye sinemiphumo emihle.[1-3]yokuthintela i-oxidation ye-membrane yebhayoloji kunye nokuzinzisa izicubu zeeseli zebhayoloji, njl.njl. Ngeminyaka yoo-1970, ingcali yezolimo yaseLatvia kwiSoviet Union yafumanisa ukuba i-diludine ineziphumo[4]yokukhuthaza ukukhula kweenkukhu kunye nokumelana nokuqandisa nokwaluphala kwezinye izityalo. Kwaxelwa ukuba i-diludine ayinakukhuthaza kuphela ukukhula kwezilwanyana, kodwa inokuphucula ukusebenza kokuzala kwezilwanyana ngokucacileyo kwaye iphucule izinga lokukhulelwa, imveliso yobisi, imveliso yamaqanda kunye nesantya sokuqanduselwa kwezilwanyana zezilwanyana zemazi.[1, 2, 5-7]Uphononongo lwe-diludine eTshayina lwaqalwa ukusukela ngeminyaka yoo-1980, kwaye uninzi lwezifundo malunga ne-diludine eTshayina zixhomekeke ekusebenziseni ukusebenza ukuza kuthi ga ngoku, kwaye kuxelwe uvavanyo olumbalwa kwiintaka ezibeka amaqanda. UChen Jufang (1993) uxele ukuba i-diludine inokuphucula imveliso yeqanda kunye nobunzima beqanda lenkukhu, kodwa ayizange inzulu.[5]uphononongo lwendlela esebenza ngayo loo nto. Ke ngoko, siqalise uphononongo olucwangcisiweyo lwempembelelo kunye nendlela esebenza ngayo ngokutya iinkukhu ezizalelayo ngokutya okune-diludine, kwaye enye inxalenye yesiphumo ngoku ixelwa ngolu hlobo lulandelayo:
Itheyibhile 1 Ulwakhiwo kunye nenxalenye yezakhamzimba zokutya kovavanyo
%
-- ...
Ukwakhiwa kokutya Izinto eziluncedo
-- ...
Umbona 62 ME③ 11.97
Impuphu yeembotyi 20 CP 17.8
Isidlo seentlanzi 3 Ca 3.42
Isidlo se-rapeseed 5 P 0.75
Isidlo samathambo 2 M et 0,43
Isidlo samatye 7.5 M kunye neCys 0.75
I-Methionine 0.1
Ityuwa 0.3
Iivithamini ezininzi① 10
Izinto ezilandelelanayo② 0.1
-- ...
① I-Multivitamin: 11mg ye-riboflavin, 26mg ye-folic acid, 44mg ye-oryzanin, 66mg ye-niacin, 0.22mg ye-biotin, 66mg ye-B6, 17.6ug ye-B12, 880mg ye-choline, 30mg ye-VK, 66IU ye-VE, 6600ICU ye-VDkunye ne-20000ICU ye-VA, zongezwa kwikhilogram nganye yokutya; kwaye i-10g ye-multivitamin yongezwa kwi-50kg nganye yokutya.
② Izinto ezilandelelanayo (mg/kg): 60 mg ye-Mn, 60 mg ye-Zn, 80 mg ye-Fe, 10 mg ye-Cu, 0.35 mg ye-I kunye ne-0.3 mg ye-Se zongezwa kwikhilogram nganye yokutya.
③ Iyunithi yamandla anyibilikiswayo ibhekisa kwi-MJ/kg.
1. Izixhobo kunye nendlela yokwenza
1.1 Izixhobo zovavanyo
I-Beijing Sunpu Biochem. & Tech. Co., Ltd. kufuneka inike i-diludine; kwaye isilwanyana esivavanyiweyo kufuneka sibhekisele kwiinkukhu zaseRoma ezizalela amaqanda ezithengiswayo ezineentsuku ezingama-300 ubudala.
Ukutya kovavanyo: ukutya kovavanyo lovavanyo kufuneka kulungiselelwe ngokwemeko yokwenyani ngexesha lokuveliswa ngokusekelwe kumgangatho we-NRC, njengoko kubonisiwe kwiTheyibhile 1.
1.2 Indlela yovavanyo
1.2.1 Uvavanyo lokutyisa: uvavanyo lokutyisa kufuneka lwenziwe kwifama yeHongji Company eJiande City; Iinkukhu ezingama-1024 zamaRoma ezibeka amaqanda kufuneka zikhethwe kwaye zahlulwe zibe ngamaqela amane ngokungacwangciswanga kwaye nganye ibe ngamaqhekeza angama-256 (iqela ngalinye kufuneka liphindwe kane, kwaye inkukhu nganye kufuneka iphindwe kane); iinkukhu kufuneka zondliwe ngezidlo ezine ezinemixholo eyahlukeneyo ye-diludine, kwaye kongezwe i-0, 100, 150, 200mg/kg yokutya kwiqela ngalinye. Uvavanyo lwaqalwa nge-10 ka-Epreli, 1997; kwaye iinkukhu zazinokufumana ukutya kwaye zisele amanzi ngokukhululekileyo. Ukutya okuthathwe liqela ngalinye, izinga lokubeka amaqanda, imveliso yeqanda, iqanda eliphukileyo kunye nenani leqanda elingaqhelekanga kufuneka lirekhodwe. Ngaphezu koko, uvavanyo lwaphela nge-30 kaJuni, 1997.
1.2.2 Ukulinganiswa komgangatho weqanda: Amaqanda angama-20 kufuneka athathwe ngokungacwangciswanga xa uvavanyo luqhutywa iintsuku ezine ezingama-40 ukuze kulinganiswe izalathisi ezinxulumene nomgangatho weqanda, ezifana nesalathisi sokuma kweqanda, iyunithi ye-haugh, ubunzima begobolondo, ubukhulu begobolondo, isalathisi somthubi, ubunzima bomthubi, njl. Ngaphezu koko, umxholo we-cholesterol kwi-yolk kufuneka ulinganiswe ngokusebenzisa indlela ye-COD-PAP phambi kwe-reagent yeCicheng eveliswe yiNingbo Cixi Biochemical Test Plant.
1.2.3 Ukulinganiswa kwe-serum biochemical index: Iinkukhu zovavanyo ezili-16 kufuneka zithathwe kwiqela ngalinye ngexesha ngalinye xa uvavanyo lwalusenziwa iintsuku ezingama-30 kwaye xa uvavanyo lugqityiwe ukulungiselela i-serum emva kokuthabatha isampuli yegazi kumthambo osephikweni. I-serum kufuneka igcinwe kubushushu obuphantsi (-20℃) ukuze kulinganiswe ii-biochemical index ezifanelekileyo. Ipesenti yamafutha esiswini kunye nomxholo we-lipid yesibindi kufuneka zilinganiswe emva kokuxhela nokukhupha amafutha esiswini kunye nesibindi emva kokugqiba ukuthatha isampuli yegazi.
I-superoxide dismutase (SOD) kufuneka ilinganiswe ngokusebenzisa indlela yokugcwala phambi kwekhithi ye-reagent eveliswe yiBeijing Huaqing Biochem. & Tech. Research Institute. I-uric acid (UN) kwi-serum kufuneka ilinganiswe ngokusebenzisa indlela ye-U ricase-PAP phambi kwekhithi ye-Cicheng reagent; i-triglyceride (TG3) kufuneka ilinganiswe ngokusebenzisa indlela ye-GPO-PAP enenyathelo elinye phambi kwekhithi ye-Cicheng reagent; i-lipase kufuneka ilinganiswe ngokusebenzisa i-nephelometry phambi kwekhithi ye-Cicheng reagent; i-cholesterol iyonke kwi-serum (CHL) kufuneka ilinganiswe ngokusebenzisa indlela ye-COD-PAP phambi kwekhithi ye-Cicheng reagent; i-glutamic-pyruvic transaminase (GPT) kufuneka ilinganiswe ngokusebenzisa i-colorimetry phambi kwekhithi ye-Cicheng reagent; i-glutamic-oxalacetic transaminase (GOT) kufuneka ilinganiswe ngokusebenzisa i-colorimetry phambi kwekhithi ye-Cicheng reagent; i-alkaline phosphatase (ALP) kufuneka ilinganiswe ngokusebenzisa indlela yokulinganisa xa kukho i-Cicheng reagent kit; i-calcium ion (Ca2+kwi-serum kufuneka ilinganiswe ngokusebenzisa i-methylthymol blue complex. Enye indlela xa kukho i-Cicheng reagent kit; i-inorganic phosphorus (P) kufuneka ilinganiswe ngokusebenzisa i-molybdate blue method xa kukho i-Cicheng reagent kit.
2 Iziphumo zovavanyo
2.1 Impembelelo ekusebenzeni kakuhle kokubeka
Ukusebenza kokubeka kwamaqela ahlukeneyo acutshungulwa kusetyenziswa i-diludine kuboniswe kwiTheyibhile 2.
Itheyibhile 2 Ukusebenza kweenkukhu ezityiswa ukutya okusisiseko okongeziweyo ngamanqanaba amane e-diludine
| Ubungakanani be-diludine obuza kongezwa (mg/kg) | ||||
| 0 | 100 | 150 | 200 | |
| Ukutya okutyiwayo (g) |  | |||
| Izinga lokubeka (%) | ||||
| Ubunzima obuqhelekileyo beqanda (g) | ||||
| Umlinganiselo wezinto ezisetyenziswa kwiqanda | ||||
| Izinga lamaqanda aphukileyo (%) | ||||
| Izinga leqanda elingaqhelekanga (%) | ||||
Ukusuka kwiTheyibhile 2, amazinga okubekwa kwamaqela onke acutshungulwa ngokusebenzisa i-diludine aphucukile ngokucacileyo, apho isiphumo xa sicutshungulwa kusetyenziswa i-150mg/kg silungile (ukuya kuthi ga kwi-83.36%), kwaye i-11.03% (p<0.01) iphucukile xa ithelekiswa neqela elibhekisayo; ngoko ke i-diludine inefuthe lokuphucula izinga lokubekwa. Xa kujongwa kubunzima obuqhelekileyo beqanda, ubunzima beqanda buyanda (p>0.05) ngokunyuka kwe-diludine ekutyeni kwemihla ngemihla. Xa kuthelekiswa neqela elibhekisayo, umahluko phakathi kwazo zonke iindawo ezicutshungulwa zamaqela acutshungulwa kusetyenziswa i-200mg/kg ye-diludine awubonakali xa i-1.79g yokutya ifakwa ngokomyinge; Nangona kunjalo, umahluko uba sobala kancinci kancinci kunye nokwanda kwe-diludine, kwaye umahluko womlinganiselo wezinto ezisetyenziswayo kwiqanda phakathi kweendawo ezisetyenziswayo uyabonakala (p<0.05), kwaye isiphumo silungile xa i-150mg/kg ye-diludine kwaye yi-1.25:1 encitshiswa yi-10.36% (p<0.01) xa kuthelekiswa neqela elibhekisa kulo. Njengoko kujongwa kwizinga lamaqanda aphukileyo kuzo zonke iindawo ezisetyenziswayo, izinga lamaqanda aphukileyo (p<0.05) linokuncipha xa i-diludine yongezwa kukutya kwemihla ngemihla; kwaye ipesenti yamaqanda angaqhelekanga iyancitshiswa (p<0.05) xa i-diludine isanda.
2.2 Impembelelo kumgangatho weqanda
Njengoko kubonwe kwiTheyibhile 3, isalathisi semilo yeqanda kunye nobunzima beqanda elithile azichaphazeleki (p>0.05) xa i-diludine yongezwa kukutya kwemihla ngemihla, kwaye ubunzima beqokobhe bunyuswa xa i-diludine yongezwa kukutya kwemihla ngemihla, apho ubunzima beqokobhe bunyuswa nge-10.58% kunye ne-10.85% (p<0.05) ngokwahlukeneyo xa kuthelekiswa namaqela ereferensi xa kongezwa i-150 kunye ne-200mg/kg ye-diludine; ubukhulu beqokobhe leqanda bunyuswa xa kongezwa i-diludine ekutyeni kwemihla ngemihla, apho ubukhulu beqokobhe leqanda bunyuswa nge-13.89% (p<0.05) xa kongezwa i-100mg/kg ye-diludine xa kuthelekiswa namaqela ereferensi, kwaye ubukhulu beqokobhe leqanda bunyuswa nge-19.44% (p<0.01) kunye ne-27.7% (p<0.01) ngokwahlukeneyo xa kongezwa i-150 kunye ne-200mg/kg. Iyunithi yeHaugh (p<0.01) iphucuka ngokucacileyo xa kongezwa i-diludine, nto leyo ebonisa ukuba i-diludine inefuthe lokukhuthaza ukwenziwa kwe-albumen etyebileyo yeqanda elimhlophe. I-diludine inomsebenzi wokuphucula isalathisi se-yolk, kodwa umahluko awubonakali ngokucacileyo (p<0.05). Umxholo we-cholesterol ye-yolk yeqanda kuwo onke amaqela uhlukile kwaye unokuncitshiswa ngokucacileyo (p<0.05) emva kokongeza i-150 kunye ne-200mg/kg ye-diludine. Ubunzima obuhambelanayo be-yolk yeqanda bahlukile omnye komnye ngenxa yezixa ezahlukeneyo ze-diludine ezongeziweyo, apho ubunzima obuhambelanayo be-yolk yeqanda buphucuka nge-18.01% kunye ne-14.92% (p<0.05) xa i-150mg/kg kunye ne-200mg/kg xa kuthelekiswa neqela elibhekisayo; ke ngoko, i-diludine efanelekileyo inefuthe lokukhuthaza ukwenziwa kwe-yolk yeqanda.
Itheyibhile 3 Imiphumo ye-diludine kumgangatho wamaqanda
| Ubungakanani be-diludine obuza kongezwa (mg/kg) | ||||
| Umgangatho weqanda | 0 | 100 | 150 | 200 |
| Isalathisi sokuma kweqanda (%) |  | |||
| Ubunzima beqanda elithile (g/cm3) | ||||
| Ubunzima obufanelekileyo beqokobhe leqanda (%) | ||||
| Ubukhulu beqokobhe leqanda (mm) | ||||
| Iyunithi yokuhleka (U) | ||||
| Isalathisi somthubi weqanda (%) | ||||
| I-cholesterol yeqanda eliyi-yolk (%) | ||||
| Ubunzima obufanelekileyo bomthubi weqanda (%) | ||||
2.3 Impembelelo kwipesenti yamafutha esiswini kunye nomxholo wamafutha esibindi kwiinkukhu ezizalelayo
Jonga uMfanekiso 1 kunye noMfanekiso 2 ukuze ufumane imiphumo ye-diludine kwipesenti yamafutha esiswini kunye nomxholo wamafutha esibindi kwiinkukhu ezizalelayo.
Umfanekiso 1 Isiphumo se-diludine kwipesenti yamafutha esisu (i-PAF) kwiinkukhu ezizalelayo
| Ipesenti yamafutha esiswini | |
| Isixa se-diludine esiza kongezwa |
Umfanekiso 2 Impembelelo ye-diludine kumthamo wamafutha esibindi (LF) kwiinkukhu ezizalelayo
| Umxholo wamafutha esibindi | |
| Isixa se-diludine esiza kongezwa |
Njengoko kubonwe kuMfanekiso 1, iipesenti zamafutha esisu kwiqela lovavanyo zincitshisiwe nge-8.3% kunye ne-12.11% (p<0.05) ngokwahlukeneyo xa i-100 kunye ne-150mg/kg ye-diludine xa kuthelekiswa neqela elibhekisayo, kwaye ipesenti yamafutha esisu incitshisiwe nge-33.49% (p<0.01) xa kongezwa i-200mg/kg ye-diludine. Njengoko kubonwe kuMfanekiso 2, umxholo wamafutha esibindi (owomileyo ngokupheleleyo) ocutshungulwe yi-100, 150, 200mg/kg ye-diludine ngokwahlukeneyo uncitshisiwe nge-15.00% (p<0.05), 15.62% (p<0.05) kunye ne-27.7% (p<0.01) ngokwahlukeneyo xa kuthelekiswa neqela elibhekisayo; ngoko ke, i-diludine inefuthe lokunciphisa ipesenti yamafutha asesiswini kunye nomxholo wamafutha esibindi kumxholo wokubeka ngokucacileyo, apho isiphumo siba ngcono xa kongezwa i-200mg/kg ye-diludine.
2.4 Impembelelo kwi-serum biochemical index
Njengoko kubonwe kwiTheyibhile 4, umahluko phakathi kwamacandelo acutshungulwayo ngexesha leSigaba soku-1 (30d) sovavanyo lwe-SOD awubonakali, kwaye ii-serum biochemical indexes zawo onke amaqela apho kongezwa khona i-diludine kwiSigaba sesi-2 (80d) sovavanyo ziphezulu kuneqela elibhekiselweyo (p<0.05). I-uric acid (p<0.05) kwi-serum inokuncipha xa kongezwa i-150mg/kg kunye ne-200mg/kg ye-diludine; ngelixa isiphumo (p<0.05) sifumaneka xa kongezwa i-100mg/kg ye-diludine kwiSigaba soku-1. I-diludine inokunciphisa i-triglyceride kwi-serum, apho isiphumo singcono (p<0.01) kwiqela xa kongezwa i-150mg/kg ye-diludine kwiSigaba soku-1, kwaye singcono kwiqela xa kongezwa i-200mg/kg ye-diludine kwiSigaba sesi-2. I-cholesterol iyonke kwi-serum iyancipha xa i-diludine yongezwa kukutya kwemihla ngemihla, ngakumbi umxholo we-cholesterol iyonke kwi-serum uncitshiswa ngama-36.36% (p<0.01) kunye nama-40.74% (p<0.01) ngokwahlukeneyo xa i-150mg/kg kunye ne-200mg/kg ye-diludine zongezwa kwiSigaba soku-1 xa kuthelekiswa neqela elibhekisayo, kwaye zincitshiswe ngama-26.60% (p<0.01), ama-37.40% (p<0.01) kunye nama-46.66% (p<0.01) ngokwahlukeneyo xa i-100mg/kg, i-150mg/kg kunye ne-200mg/kg ye-diludine zongezwa kwiSigaba sesi-2 xa kuthelekiswa neqela elibhekisayo. Ngaphezu koko, i-ALP iyanda xa i-diludine yongezwa kukutya kwemihla ngemihla, ngelixa amaxabiso e-ALP kwiqela apho kongezwa khona i-150mg/kg kunye ne-200mg/kg ye-diludine aphezulu kuneqela elibhekiselweyo (p<0.05) ngokucacileyo.
Itheyibhile 4 Iziphumo ze-diludine kwiiparameter ze-serum
| Ubungakanani be-diludine obuza kongezwa (mg/kg) kwiSigaba sokuQala (30d) sovavanyo | ||||
| Into | 0 | 100 | 150 | 200 |
| I-Superoxide dismutase (mg/mL) |  | |||
| I-asidi ye-Uric | ||||
| I-Triglyceride (mmol/L) | ||||
| I-Lipase (U/L) | ||||
| I-Cholesterol (mg/dL) | ||||
| I-transaminase ye-Glutamic-pyruvic (U/L) | ||||
| I-transaminase ye-Glutamic-oxalacetic (U/L) | ||||
| I-alkaline phosphatase (mmol/L) | ||||
| I-calcium ion (mmol/L) | ||||
| I-phosphorus engaphiliyo (mg/dL) | ||||
| Ubungakanani be-diludine obuza kongezwa (mg/kg) kwiSigaba sesiBini (80d) sovavanyo | ||||
| Into | 0 | 100 | 150 | 200 |
| I-Superoxide dismutase (mg/mL) |  | |||
| I-asidi ye-Uric | ||||
| I-Triglyceride (mmol/L) | ||||
| I-Lipase (U/L) | ||||
| I-Cholesterol (mg/dL) | ||||
| I-transaminase ye-Glutamic-pyruvic (U/L) | ||||
| I-transaminase ye-Glutamic-oxalacetic (U/L) | ||||
| I-alkaline phosphatase (mmol/L) | ||||
| I-calcium ion (mmol/L) | ||||
| I-phosphorus engaphiliyo (mg/dL) | ||||
3 Uhlalutyo kunye nengxoxo
3.1 I-diludine kuvavanyo iphucule izinga lokubekwa kweqanda, ubunzima beqanda, iyunithi yeHaugh kunye nobunzima obuhambelanayo beqanda, nto leyo ebonisa ukuba i-diludine inefuthe lokukhuthaza ukufunxwa kweproteni kunye nokuphucula ubungakanani bokwenziwa kwe-albumen etyebileyo yeqanda elimhlophe kunye neproteni yeqanda elimhlophe. Ngaphezu koko, umxholo we-uric acid kwi-serum uncitshisiwe ngokucacileyo; kwaye ngokubanzi kwavunywa ukuba ukunciphisa umxholo we-nitrogen engeyiyo iproteni kwi-serum kuthetha ukuba isantya se-catabolism yeproteni sinciphile, kwaye ixesha lokugcina i-nitrogen lihlehlisiwe. Esi siphumo sabonelela ngesiseko sokwandisa ukugcinwa kweproteni, ukukhuthaza ukubekwa kwamaqanda kunye nokuphucula ubunzima beqanda leenkuku ezibeka amaqanda. Isiphumo sovavanyo sabonisa ukuba isiphumo sokubekwa kweqanda silungile xa kongezwa i-150mg/kg ye-diludine, nto leyo eyayihambelana nesiphumo.[6,7]kaBao Erqing kunye noQin Shangzhi kwaye yafunyanwa ngokongeza i-diludine ngexesha lokugqibela lokubeka iinkukhu. Isiphumo sancitshiswa xa ubungakanani be-diludine budlula i-150mg/kg, okusenokuba kungenxa yokuguqulwa kweproteni[8]yachaphazeleka ngenxa yedosi egqithisileyo kunye nomthwalo ogqithisileyo wemetabolism yesitho ukuya kwi-diludine.
3.2 Uxinzelelo lweCa2+kwi-serum yeqanda elizalelayo lancitshiswa, i-P kwi-serum yancitshiswa ekuqaleni kwaye umsebenzi we-ALP wanda ngokucacileyo xa kukho i-diludine, nto leyo ebonisa ukuba i-diludine ichaphazele imetabolism ye-Ca kunye ne-P ngokucacileyo. UYue Wenbin uxele ukuba i-diludine inokukhuthaza ukufunxwa.[9] izinto zeminerali iFe kunye neZn; I-ALP yayikhona kakhulu kwizicubu, ezifana nesibindi, ithambo, indlela yamathumbu, izintso, njl.njl.; I-ALP kwi-serum yayivela kwisibindi namathambo ikakhulu; I-ALP kwithambo yayikhona kakhulu kwi-osteoblast kwaye yayinokudibanisa i-phosphate ion kunye ne-Ca2 evela kwi-serum emva kotshintsho ngokukhuthaza ukubola kwe-phosphate kunye nokwandisa uxinzelelo lwe-phosphate ion, kwaye yafakwa kwithambo ngohlobo lwe-hydroxyapatite, njl.njl. ukuze kukhokelele ekunciphiseni i-Ca kunye ne-P kwi-serum, okuhambelana nokwanda kobukhulu begobolondo leqanda kunye nobunzima obuhambelanayo begobolondo leqanda kwizalathisi zomgangatho weqanda. Ngaphezu koko, izinga leqanda elaphukileyo kunye nepesenti yeqanda elingaqhelekanga zancitshiswa ngokucacileyo ngokwendlela yokusebenza kokubeka, nto leyo ekwachaza le ngongoma.
3.3 Ukubekwa kwamafutha esiswini kunye nomxholo wamafutha esibindi kwiinkukhu ezizalelayo kuncitshisiwe ngokucacileyo ngokongeza i-diludine ekutyeni, nto leyo ebonisa ukuba i-diludine inefuthe lokuthintela ukwenziwa kwamafutha emzimbeni. Ngaphezu koko, i-diludine inokuphucula umsebenzi we-lipase kwi-serum kwinqanaba lokuqala; umsebenzi we-lipase wonyuswe ngokucacileyo kwiqela apho kongezwe khona i-100mg/kg ye-diludine, kwaye umxholo we-triglyceride kunye ne-cholesterol kwi-serum wehlisiwe (p<0.01), nto leyo ebonisa ukuba i-diludine inokukhuthaza ukubola kwe-triglyceride kwaye ithintele ukwenziwa kwe-cholesterol. Ukubekwa kwamafutha kunokuthintelwa kuba i-enzyme ye-lipid metabolism kwisibindi.[10,11], kunye nokunciphisa i-cholesterol kwiqanda leqanda kuchaze eli nqaku [13]. UChen Jufang uxele ukuba i-diludine inokuthintela ukwakheka kwamafutha kwisilwanyana kwaye iphucule ipesenti yenyama engenamafutha kwiinkukhu zenyama kunye neehagu, kwaye inefuthe lokunyanga isibindi esinamafutha. Iziphumo zovavanyo zicacise le ndlela yokusebenza, kwaye iziphumo zokuqhekeka kunye nokubonwa kweenkukhu zovavanyo zibonakalise ukuba i-diludine inokunciphisa izinga lokuvela kwesibindi esinamafutha kwiinkukhu ezibeka amaqanda ngokucacileyo.
3.4 I-GPT kunye ne-GOT ziimpawu ezimbini ezibalulekileyo ezibonisa imisebenzi yesibindi nentliziyo, kwaye isibindi nentliziyo zinokonakala ukuba imisebenzi yazo iphezulu kakhulu. Imisebenzi ye-GPT kunye ne-GOT kwi-serum ayizange itshintshe ngokucacileyo xa i-diludine yongezwa kuvavanyo, olubonise ukuba isibindi nentliziyo azonakalanga; ngaphezulu koko, isiphumo sokulinganisa se-SOD sibonise ukuba umsebenzi we-SOD kwi-serum unokuphuculwa ngokucacileyo xa i-diludine isetyenziswa ixesha elithile. I-SOD ibhekisa kwi-scavenger enkulu ye-superoxide free radical emzimbeni; ibalulekile ekugcineni ukuthembeka kwe-membrane yebhayoloji, ukuphucula amandla okuzikhusela komzimba kunye nokugcina impilo yesilwanyana xa umxholo we-SOD emzimbeni unyusiwe. UQuh Hai, njl. njl. baxele ukuba i-diludine inokuphucula umsebenzi we-6-glucose phosphate dehydrogenase kwi-membrane yebhayoloji kwaye izinzise izicubu [2] zeseli yebhayoloji. USniedze ubonise ukuba i-diludine ithintele umsebenzi [4] we-NADPH cytochrome C reductase ngokucacileyo emva kokufunda ubudlelwane phakathi kwe-diludine kunye ne-enzyme efanelekileyo kwi-NADPH specific electron transfer chain kwi-rat liver microsome. I-Odydents ikwabonise ukuba i-diludine yayinxulumene [4] nenkqubo ye-composite oxidase kunye ne-microsomal enzyme enxulumene ne-NADPH; kwaye indlela yokusebenza kwe-diludine emva kokungena kwisilwanyana kukudlala indima yokuchasana ne-oxidation kunye nokukhusela i-biological membrane [8] ngokuthintela umsebenzi we-electron transfer NADPH enzyme ye-microsome kunye nokuthintela inkqubo ye-peroxidation ye-lipid compound. Iziphumo zovavanyo zibonakalise ukuba umsebenzi wokukhusela we-diludine kwi-biological membrane ukusuka kutshintsho lomsebenzi we-SOD ukuya kutshintsho lwemisebenzi ye-GPT kunye ne-GOT kwaye zibonakalise iziphumo zophando zeSniedze kunye ne-Odydents.
Isalathiso
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2 Qu Hai, Lv Ye, Wang Baosheng, Isiphumo se-diludine songezwa kukutya kwemihla ngemihla kwinqanaba lokukhulelwa kunye nomgangatho wembewu yemvundla yenyama.Ijenali kaJ. Chinese yezolimo lweeMvundla1994(6): 6-7
3 Chen Jufang, Yin Yuejin, Liu Wanhan, njl. Uvavanyo lokusetyenziswa okwandisiweyo kwe-diludine njengesongezelelo sokutyaUphando lwezondlo1993 (3): 2-4
4 UZheng Xiaozhong, uLi Kelu, uYue Wenbin, njl. Ingxoxo ngesiphumo sokusetyenziswa kunye nendlela yokusebenza kwe-diludine njengomkhuthazi wokukhula kwenkukhuUphando lwezondlo1995 (7): 12-13
5 Chen Jufang, Yin Yuejin, Liu Wanhan, njl. Uvavanyo lokusetyenziswa okwandisiweyo kwe-diludine njengesongezelelo sokutyaUphando lwezondlo1993 (3): 2-5
6 Bao Erqing, Gao Baohua, Uvavanyo lwediludine yokondla inzala yasePekingUphando lwezondlo1992 (7): 7-8
7 Uvavanyo lweQin Shangzhi lokuphucula imveliso yeenkukhu zenyama zohlobo lwazo ekupheleni kwexesha lokuzala ngokusebenzisa i-diludineIjenali yeGuangxi yoBufuyo lweZilwanyana kunye neMithi yeZilwanyana1993.9(2): 26-27
8 Dibner J Jl Lvey FJ Iprotheni yesibindi kunye ne-amino acid metabolian kwinkukhu Isayensi yeenkukhu1990.69(7): 1188-1194
9 Yue Wenbin, Zhang Jianhong, Zhao Peie, njl. Uphononongo lokongeza i-diludine kunye nokulungiselela i-Fe-Zn ekutyeni kwemihla ngemihla kweenkukhu ezibeka amaqanda.Ukutya kunye nemfuyo1997, 18(7): 29-30
10 Mildner A na M, Steven D Clarke I-Porcine fatty acid synthase cloning ye-DNA eyongezelelekileyo, ukusasazwa kwezicubu ze-itsmRNA kunye nokunciphisa ukubonakaliswa yi-somatotropin kunye neproteni yokutya J Nutri 1991, 121 900
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12 Donaldson WE Umetabolism ye-lipid kwimpendulo yesibindi seentsana xa zisityaIsayensi yeenkukhu. 1990, 69(7): 1183-1187
13 Ksiazk ieu icz J. K ontecka H, H ogcw sk i L Inqaku malunga ne-cholesterol yegazi njengophawu lokutyeba emzimbeni kumadadaIjenali yeSayensi ye-Aninal kunye ne-Feed,1992, 1(3/4): 289-294
Ixesha lokuthumela: Juni-07-2021
