I-Tributyrin (TB)kwayeI-Monolaurin (GML), njengezongezo zokutya ezisebenzayo, zineziphumo ezininzi zomzimba ekukhuliseni iinkukhu ezisemaqeleni, ziphucula kakhulu ukusebenza kwemveliso yamaqanda, umgangatho wamaqanda, impilo yamathumbu, kunye ne-lipid metabolism. Nazi imisebenzi yazo ephambili kunye neendlela:

1. Ukuphucula ukusebenza kwemveliso yamaqanda
I-Glycerol Monolaurate(GML)

Ukongeza i-0.15-0.45g/kg ye-GML ekutyeni kweenkukhu ezizalelayo kunokunyusa kakhulu izinga lokuvelisa amaqanda, kunciphise izinga lokuguqulwa kokutya, kwaye konyuse ubunzima beqanda obuqhelekileyo.
Uphononongo lubonisa ukuba i-300-450mg/kg ye-GML inokuphucula izinga lokuvelisa amaqanda kwiinkukhu ezizalelayo kwaye inciphise izinga lamaqanda aneengxaki.

I-Tributyrin (TB) 

Kuvavanyo lweenkukhu ze-broiler, i-500mg/kg ye-TB inokulibazisa ukwehla kwesantya sokuvelisa amaqanda kwinqanaba lokugqibela lokubeka amaqanda, iphucule amandla eqokobhe lamaqanda, kwaye inciphise izinga lokuqandusela.
Idityaniswe neI-GML(njengefomula enelungelo lobunikazi) inokwandisa ixesha lokuvelisa amaqanda aphezulu kwaye iphucule iingenelo zoqoqosho.

2. Phucula umgangatho wamaqanda

Umsebenzi we-GML
Yandisa ubude beproteni, iiyunithi zeHaff (HU), kwaye uphucule umbala womthubi.
Lungisa ukwakheka kwe-fatty acid kwi-egg yolk, wandise i-polyunsaturated fatty acids (PUFA) kunye ne-monounsaturated fatty acids (MUFA), kwaye unciphise umxholo we-saturated fatty acids (SFA).

Ngedosi ye-300mg/kg, i-GML yonyuse kakhulu ubulukhuni beqokobhe leqanda kunye nomxholo weproteni emhlophe yeqanda.

Umsebenzi weTB

Yandisa amandla eeqokobhe zamaqanda kwaye unciphise izinga lokuqhekeka kweqokobhe (njengokunciphisa i-58.62-75.86% kwiimvavanyo).

Khuthaza ukubonakaliswa kwezakhi zofuzo ezinxulumene nokugcinwa kwekhalsiyam yesibeleko (njenge-CAPB-D28K, OC17) kwaye uphucule i-calcification yegobolondo leqanda.

3. Ukulawula imetabolism yamafutha kunye nomsebenzi we-antioxidant
Umsebenzi we-GML
Nciphisa i-serum triglycerides (TG), i-cholesterol iyonke (TC), kunye ne-low-density lipoprotein cholesterol (LDL-C), kwaye unciphise ukugcinwa kwamafutha esiswini.
Phucula umsebenzi we-serum superoxide dismutase (SOD) kunye ne-glutathione peroxidase (GSH Px), nciphisa umxholo we-malondialdehyde (MDA), kwaye wonyuse amandla e-antioxidant.
Umsebenzi weTB
Yehlisa umxholo we-triglyceride yesibindi (10.2-34.23%) kwaye wonyuse ii-genes ezinxulumene ne-oxidation yamafutha (ezifana ne-CPT1).
Yehlisa amanqanaba e-serum alkaline phosphatase (AKP) kunye ne-MDA, kwaye yonyuse umthamo we-total antioxidant (T-AOC).

4. Ukuphucula impilo yamathumbu
Umsebenzi we-GML
Yandisa ubude be-villus kunye nomlinganiselo we-villus kwi-villus (V/C) ye-jejunum ukuphucula imo yamathumbu.
Nciphisa izinto ezibangela ukudumba (ezifana ne-IL-1 β, i-TNF-α), nyusa izinto ezilwa nokudumba (ezifana ne-IL-4, i-IL-10), kwaye uphucule umsebenzi wokuthintela amathumbu.
Yenza ngcono ulwakhiwo lwe-cecal microbiota, unciphise umlinganiselo weProteobacteria, kwaye ukhuthaze ukukhula kweebhaktheriya eziluncedo ezifana neSpirogyraceae.
Umsebenzi weTB
Lungisa ixabiso le-pH yamathumbu, khuthaza ukwanda kweebhaktheriya eziluncedo (ezifana ne-lactobacilli), kwaye uthintele iibhaktheriya eziyingozi.
Ukwanda kweproteni ye-tight junction (efana ne-Occludin, i-CLDN4) ukubonakaliswa kwezakhi zofuzo kuphucula ukuthembeka komqobo wamathumbu.

5. Impembelelo yokulawula amajoni omzimba
Umsebenzi we-GML
Ukuphucula i-spleen index kunye ne-thymus index, kuphucula ukusebenza komzimba.
Nciphisa iimpawu zokuvuvukala kwi-serum ezifana ne-aspartate aminotransferase (AST) kunye ne-alanine aminotransferase (ALT).
Umsebenzi weTB
Nciphisa impendulo yokudumba kwamathumbu ngokulawula indlela ye-Toll-like receptor (TLR2/4).

6. Isiphumo sokusebenza ngokudibeneyo
Uphando lwepatenti lubonise ukuba indibaniselwano ye-GML kunye ne-TB (ezifana ne-20-40 TB+15-30 GML) inokuphucula ngokudibeneyo izinga lokuveliswa kwamaqanda ezinkukhu ezibeka amaqanda (92.56% vs. 89.5%), inciphise ukudumba kwetyhubhu, kwaye yandise ixesha lokuveliswa kwamaqanda aphezulu.

Isishwankathelo:

IGlycerol Monolaurate (GML)kwayeI-Tributyrin (TB)zineziphumo ezongezelelweyo ekufuyeni iinkukhu:

I-GMLigxile kwiukuphucula umgangatho weqanda, ukulawula imetabolism yamafutha, kunye nomsebenzi we-antioxidant;
TBigxile kwiukuphucula impilo yamathumbu kunye ne-calcium metabolism;
Indibaniselwano inokubazisebenza ngokubambisana, ziphucula ngokupheleleyo ukusebenza kwemveliso kunye nomgangatho wamaqanda.